ELISA
– RIDASCREEN®FAST Folsäure (Folic acid)
RIDASCREEN®FAST Folsäure (Folic acid)
Art. No.: R3203
Intended Use
RIDASCREEN®FAST Folsäure (Folic Acid) is a competitive enzyme immunoassay for the quantitative determination of added folic acid in milk, milk powder, food for special medical purpose, grain and cereals, fortified flour, vitamin powder, -mixture, -tablets, and vitamin juice.
General Information
Folic acid also known as vitamin B9 or vitamin B11 belongs to the group of water soluble vitamins. It can´t be produced by the human body and therefore must be included in the diet in order to meet the daily requirements. Folic acid plays a key role in all growth and cell division processes. Adequate folic acid intake during pregnancy is important to avoid neural tube defects, for example. Some countries have agreed to fortify either bread, cereals, flour, pasta, rice or other grain products with folic acid in different concentrations as a preventive approach to improve micronutrient status of the population.
Specifications
| Art. No. | R3203 |
| Test Format | Microtiter plate with 48 wells (6 strips with 8 removable wells each) |
| Sample preparation | milk: can be applied directly, dilution if necessary juices: can be applied directly, dilution if necessary other matrices: extraction, dilution if necessary |
| Incubation Time | 25 min |
| Detection Limit | 0.5 μg/L (ppb)
(corresponding to standard substance) |
| Cross reactivity | Folic Acid: 100 % Dihydrofolic Acid: approx. 14 % Tetrahydrofolic Acid: < 0.5 % 5-Methyltetrahydrofolic Acid: < 0.5 % Formyltetrahydrofolic Acid: < 0.5 % Tetrahydrobiopterin:< 0.5 % 6-Biopterin: < 0.5 % |
Contact Info
E-mail: info@chakmartin.com
Tel: 02-559-3936
Mobile: 089-892-2997
Vitamin B12
– RIDASCREEN®FAST Vitamin B12
RIDASCREEN®FAST Vitamin B12
Art. No.: R2103
Intended Use
RIDASCREEN®FAST Vitamin B12 is a competitive enzyme immunoassay for the quantitative determination of Vitamin B12 in milk, milk powder, food for special medical purpose, grain and cereals, fortified flour, vitamin powder, -mixture, -tablets, and vitamin juice.
General Information
Vitamin B12 belongs to group of the water soluble vitamins. It is not produced by the human body and must be included in the diet. Vitamin B12 is needed only in small concentrations, however it plays an important role for the nervous system, has protective effects for the heart and plays an important role in nucleotide biosynthesis, iron intake, and metabolism of amino acids. A lack of vitamin B12 can lead to anemia and neurological deficits.
Specifications
| Art. No. | R2103 |
| Test Format | Microtiter plate with 48 wells (6 strips with 8 removable wells each) |
| Sample preparation | milk: can be applied directly, dilution if necessary juices: can be applied directly, dilution if necessary other matrices: extraction, dilution if necessary |
| Incubation Time | 25 min |
| Detection Limit | 0.5 μg/L (ppb)
(corresponding to standard substance) |
| Cross reactivity | Vitamin B12 (Cyanocobalamin) 100% Hydroxocobalamin approx. 95% Methylcobalamin approx. 95% Adenosylcobalamin (Coenzym B12) approx. 91% |
Contact Info
E-mail: info@chakmartin.com
Tel: 02-559-3936
Mobile: 089-892-2997
Biotin
– VitaFast® Vitamin B7 (Biotin) AOAC-RI (101001)
VitaFast® Vitamin B7 (Biotin) AOAC-RI (101001)
Art. No.: P1003
Intended Use
Easy to use microbiological microtiter plate test for the quantification of total biotin (enriched and natural biotin) in food, feed and pharmaceutical products.
General information
Biotin is extracted from the sample and the extract is diluted. The diluted extract and the biotin assay-medium are pipetted into the wells of a microtiter plate which is coated with Lactobacillus plantarum. The growth of Lactobacillus plantarum is dependent on the supply of biotin. Following the addition of biotin as a standard or as a compound of the sample, the bacteria grow until the vitamin is consumed. The incubation is done in the dark at 37 °C (98.6 °F) for 44 – 48 h. The intensity of metabolism or growth in relation to the extracted biotin is measured as turbidity and compared to a standard curve. The measurement is done using an microtiter plate spectrophotometer at 610 – 630 nm (alternatively at 540 – 550 nm).
Accessories
VitaFast® Vitamin B7 (Biotin) Dotierstandard/Spiking Standard
Certificates
AOAC-RI – 101001
Specifications
Art. No.: P1003
Test format: Microtiter plate with 96 wells (12 strips with 8 removable wells each)
Incubation time : 44 – 48 h in the dark at 37 °C (98.6 °F)
Contact Info
E-mail: info@chakmartin.com
Tel: 02-559-3936
Mobile: 089-892-2997
Pantothenic Acid
– VitaFast® Folsäure / Folic Acid AOAC-RI (100903)
VitaFast® Folsäure / Folic Acid AOAC-RI (100903)
Intended Use
Easy to use microbiological microtiter plate test for the quantification of total folic acid (enriched and natural folic acid) in food, feed and pharmaceutical products.
General Information
Folic acid is extracted from the sample and the extract is diluted. The diluted extract and the folic acid assay-medium are pipetted into the wells of a microtiter plate which is coated with Lactobacillus rhamnosus. The growth of Lactobacillus rhamnosus is dependent on the supply of folic acid. Following the addition of folic acid as a standard or as a compound of the sample, the bacteria grow until the vitamin is consumed. The incubation is done in the dark at 37 °C (98.6 °F) for 44 – 48 h. The intensity of metabolism or growth in relation to the extracted folic acid is measured as turbidity and compared to a standard curve. The measurement is done using an microtiter plate spectrophotometer at 610 – 630 nm (alternatively at 540 – 550 nm).
Accessories
– VitaFast® Folsäure/Folic Acid Dotierstandard/Spiking Standard
– Chicken Pancreatin (γ-Glutamylhydrolase)
Certificates
AOAC-RI – 100903
Specifications
Art. No.: P1001
Test format: Microtiter plate with 96 wells (12 strips with 8 removable wells each)
Incubation time: 44 – 48 h in the dark at 37 °C (98.6 °F)
Contact Info
E-mail: info@chakmartin.com
Tel: 02-559-3936
Mobile: 089-892-2997
Vitamin B12
– VitaFast® Vitamin B12 (Cyanocobalamin) (AOAC-RI)
VitaFast® Vitamin B12 (Cyanocobalamin) (AOAC-RI)
Art. No.: P1002
Intended Use
Easy to use microbiological microtiter plate test for the quantification of total vitamin B12 (enriched and natural vitamin B12) in food, feed and pharmaceutical products.
General Information
Vitamin B12 is extracted from the sample and the extract is diluted. The diluted extract and the vitamin B12 assay-medium are pipetted into the wells of a microtiter plate which is coated with Lactobacillus delbrueckii subsp. lactis (leichmannii). The growth of Lactobacillus delbrueckii is dependent on the supply of vitamin B12. Following the addition of vitamin B12 as a standard or as a compound of the sample, the bacteria grow until the vitamin is consumed. The incubation is done in the dark at 37 °C (98.6 °F) for 44 – 48 h. The intensity of metabolism or growth in relation to the extracted vitamin B12 is measured as turbidity and compared to a standard curve. The measurement is done using an microtiter plate spectrophotometer at 610 – 630 nm (alternatively at 540 – 550 nm).
Accessories
VitaFast® Vitamin B12 (Cyanocobalamin) Dotierstandard/Spiking Standard
Certificates
AOAC-RI – 101002
Specifications
Art. No.: P1002
Test format: Microtiter plate with 96 wells (12 strips with 8 removable wells each)
Incubation time: 44 – 48 h in the dark at 37 °C (98.6 °F)
Contact Info
E-mail: info@chakmartin.com
Tel: 02-559-3936
Mobile: 089-892-2997
Folic Acid
– VitaFast® Folsäure / Folic Acid AOAC-RI (100903)
VitaFast® Folsäure / Folic Acid AOAC-RI (100903)
Art. No.: P1001
Intended Use
Easy to use microbiological microtiter plate test for the quantification of total folic acid (enriched and natural folic acid) in food, feed and pharmaceutical products.
General Information
Folic acid is extracted from the sample and the extract is diluted. The diluted extract and the folic acid assay-medium are pipetted into the wells of a microtiter plate which is coated with Lactobacillus rhamnosus. The growth of Lactobacillus rhamnosus is dependent on the supply of folic acid. Following the addition of folic acid as a standard or as a compound of the sample, the bacteria grow until the vitamin is consumed. The incubation is done in the dark at 37 °C (98.6 °F) for 44 – 48 h. The intensity of metabolism or growth in relation to the extracted folic acid is measured as turbidity and compared to a standard curve. The measurement is done using an microtiter plate spectrophotometer at 610 – 630 nm (alternatively at 540 – 550 nm).
Accessories
VitaFast® Folsäure/Folic Acid Dotierstandard/Spiking Standard
Chicken Pancreatin (γ-Glutamylhydrolase)
Certificates
AOAC-RI – 100903
Specifications
Art. No. : P1001
Test format: Microtiter plate with 96 wells (12 strips with 8 removable wells each)
Incubation time : 44 – 48 h in the dark at 37 °C (98.6 °F)
Contact Info
E-mail: info@chakmartin.com
Tel: 02-559-3936
Mobile: 089-892-2997
Pyridoxin
– VitaFast® Vitamin B6 (Pyridoxin)
VitaFast® Vitamin B6 (Pyridoxin)
Art. No.: P1008
Intended Use
Easy to use microbiological microtiter plate test for the quantification of total vitamin B6 (enriched and natural vitamin B6) in food, feed and pharmaceutical products.
General Information
Vitamin B6 is extracted from the sample and the extract is diluted. The diluted extract and the vitamin B6 assay-medium are pipetted into the wells of a microtiter plate which is coated with Saccharomyces cerevisiae. The growth of Saccharomyces cerevisiae is dependent on the supply of vitamin B6. Following the addition of vitamin B6 as a standard or as a compound of the sample, the bacteria grow until the vitamin is consumed. The incubation is done in the dark at 30 °C (86 °F) for 44 – 48 h. The intensity of metabolism or growth in relation to the extracted vitamin B6 is measured as turbidity and compared to a standard curve. The measurement is done using a microtiter plate spectrophotometer at 610 – 630 nm (alternatively at 540 – 550 nm).
Specifications
Art. No.: P1008
Test format: Microtiter plate with 96 wells (12 strips with 8 removable wells each)
Incubation time: 44 – 48 h in the dark at 30 °C (86 °F)
Contact Info
E-mail: info@chakmartin.com
Tel: 02-559-3936
Mobile: 089-892-2997
Vitamin C
– VitaFast® Vitamin C (L-Ascorbinsäure / L-Ascorbic Acid)
VitaFast® Vitamin C (L-Ascorbinsäure / L-Ascorbic Acid)
Art. No.: P1010
Intended Use
Easy to conduct test in microtiter plate format for the quantitative determination of Vitamin C in food, pharmaceutical products and other sample material. Additionally L-dehydroascorbic acid can be detected (see application note).
General Information
This is an enzymatic test in microtiter plate format for the quantitative determination of vitamin C (L-ascorbic acid) in food, pharmaceutical products and other sample materials. Additionally L-dehydroascorbic acid can be detected (see application note). The kit contains all required reagents including the L-ascorbic acid standard for the standard curve. 50 reactions (plus four calibration curves) can be carried out. For evaluation a microtiter plate photometer (550 – 570 nm) is necessary. The limit of detection and the limit of quantification of the VitaFast® Vitamin C test is 7.8 mg/100g (ml). The recovery rate ranges between 95 – 105%.
Specifications
| Art. No. | P1010 |
| Test Format | Microtiter plate with 96 wells. |
| Sample preparation | Homogenisation, extraction, filtration, dilution |
| Incubation Time | first: 20 min at 20 – 25 degree second: 30 min at 20 – 25 degree |
| Detection Limit | 7.8 mg/100 g (= quantification limit) |
Contact Info
E-mail: info@chakmartin.com
Tel: 02-559-3936
Mobile: 089-892-2997
Inositol
– VitaFast® Inositol
VitaFast® Inositol
Art. No.: P1009
Intended use
Easy to use microbiological microtiter plate test for the quantification of total inositol (added and native inositol) in food, animal feed and pharmaceutical products.
General information
Inositol is extracted from the sample and the extract is diluted. The diluted extract and the inositol assay – medium are pipetted into the wells of a microtiter plate which is coated with Saccharomyces cerevisiae. The growth of Saccharomyces cerevisiae is dependent on the supply of inositol. Following the addition of inositol as a standard or as a compound of the sample, the bacteria grow until the inositol is consumed. The incubation is done in the dark at 30 °C (86 °F) for 44 – 48 h. The intensity of metabolism or growth of Saccharomyces cerevisiae in relation to the extracted inositol is measured as turbidity and compared to a standard curve. The measurement is done using a microtiter plate spectrophotometer at 610 – 630 nm (alternatively at 540 – 550 nm).
Specifications
| Art. No. | P1009 |
| Test Format | Microtiter plate with 96 wells (12 strips with 8 removable wells each) |
| Sample preparation | For the determination of total inositol content, the sample has to be treated by enzymatic extraction |
| Incubation Time | 44 – 48 h in the dark at 30 °C (86 °F) |
Contact Info
E-mail: info@chakmartin.com
Tel: 02-559-3936
Mobile: 089-892-2997
Riboflavin
– VitaFast® Vitamin B2 (Riboflavin) (AOAC-RI)
VitaFast® Vitamin B2 (Riboflavin) (AOAC-RI)
Art. No.: P1007
Intended use
Easy to use microbiological microtiter plate test for the quantification of total vitamin B2 (enriched and natural vitamin B2) in food, feed and pharmaceutical products.
General information
Vitamin B2 is extracted from the sample and the extract is diluted. The diluted extract and the vitamin B2 assay-medium are pipetted into the wells of a microtiter plate which is coated with Lactobacillus rhamnosus. The growth of Lactobacillus rhamnosus is dependent on the supply of vitamin B2. Following the addition of vitamin B2 as a standard or as a compound of the sample, the bacteria grow until the vitamin is consumed. The incubation is done in the dark at 37 °C (98.6 °F) for 44 – 48 h. The intensity of metabolism or growth in relation to the extracted vitamin B2 is measured as turbidity and compared to a standard curve. The measurement is done using a microtiter plate spectrophotometer at 610 – 630 nm (alternatively at 540 – 550 nm).
Certificates
AOAC-RI – 100902
Specifications
| Art. No. | P1007 |
| Test Format | Microtiter plate with 96 wells (12 strips with 8 removable wells each) |
| Sample preparation | For the determination of added and total vitamin B2 the sample has to be treated with enzymes and thereafter heat extracted. |
| Incubation Time | 44 – 48 h in the dark at 30 °C (86 °F) |
Contact Info
E-mail: info@chakmartin.com
Tel: 02-559-3936
Mobile: 089-892-2997
Niacin
– VitaFast® Vitamin B3 (Niacin)
VitaFast® Vitamin B3 (Niacin)
Art. No.: P1004
Intended Use
Easy to use microbiological microtiter plate test for the quantification of total niacin (enriched and natural niacin) in food, feed and pharmaceutical products. All required reagents and standard are contained in the test kit.
General Information
Niacin is extracted from the sample and the extract is diluted. The niacin assay – medium and the diluted extract are pipetted into the wells of a microtiter plate which is coated with Lactobacillus plantarum. The growth of Lactobacillus plantarum is dependent on the supply of niacin. Following the addition of niacin as a standard or as a compound of the sample, the bacteria grow until the vitamin is consumed. The incubation is done in the dark at 37 °C (98.6 °F) for 44 – 48 h. The intensity of metabolism or growth of Lactobacillus plantarum in relation to the extracted niacin is measured as turbidity and compared to a standard curve. The measurement is done using an microtiter plate spectrophotometer at 610 – 630 nm (alternatively at 540 – 550 nm).
Specifications
Art. No.: P1004
Test format: Microtiter plate with 96 wells (12 strips with 8 removable wells each)
Incubation time: 44 – 48 h in the dark at 37 °C (98.6 °F)
Contact Info
E-mail: info@chakmartin.com
Tel: 02-559-3936
Mobile: 089-892-2997
Thiamin
– VitaFast® Vitamin B1 (Thiamin)
VitaFast® Vitamin B1 (Thiamin)
Art. No.: P1006
Intended Use
Easy to use microbiological microtiter plate test for the quantification of total vitamin B1 (enriched and natural vitamin B1) in food, feed and pharmaceutical products.
General Information
Vitamin B1 is extracted from the sample and the extract is diluted. The diluted extract and the vitamin B1-assay-medium are pipetted into the wells of a microtiter plate which is coated with Lactobacillus fermentum. The growth of Lactobacillus fermentum is dependent on the supply of vitamin B1. Following the addition of vitamin B1 as a standard or as a compound of the sample, the bacteria grows until the vitamin is consumed. The incubation is done in the dark at 37 °C (98.6 °F) for 44-48 h. The intensity of metabolism or growth in relation to the extracted vitamin B1 is measured as turbidity and compared to a standard curve. The measurement is done using a microtiter plate spectrophotometer at 610 – 630 nm (alternatively at 540 – 550 nm).
Specifications
Art. No.: P1006
Test format: Microtiter plate with 96 wells (12 strips with 8 removable wells each)
Incubation time: 44-48 h in the dark at 37 °C (98.6 °F)
Contact Info
E-mail: info@chakmartin.com
Tel: 02-559-3936
Mobile: 089-892-2997
Biotin
EASI-EXTRACT® BIOTIN
Art. No.: P82 / P82B
Intended Use
Immunoaffinity columns for use in conjuntion with an HPLC or LC-MS/MS system for detection of biotin in a wide range of commodities.
General Information
The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. Improved clean-up and concentration of toxins from complex food matrices results in reduced chromatography interference and lower detection limits.
The columns contain a gel suspension of monoclonal antibody specific to the vitamin of interest. Following extraction of the vitamin the sample extract is diluted with buffer, centrifuged and the supernatant filtered before being passed slowly through the immunoaffinity column. Any vitamin which is present in the sample is retained by the antibody within the gel suspension. The column is washed to remove unbound material and the vitamin is then released following elution with solvent. The eluate is collected, evaporated and reconstituted prior to analysis by HPLC or LC-MS/MS.
Certificates
- AOAC Official First Action Method – 2016.02
Specifications
Art. No.: P82 / P82B
Test Format: 10 wide format immunoaffinity columns (P82).
50 wide format immunoaffinity columns (P82B).
Detection Limit: LOD: 25 ng/ml
Contact Info
E-mail: info@chakmartin.com
Tel: 02-559-3936
Mobile: 089-892-2997
– EASI-EXTRACT® MULTI-VIT B (LGE)
EASI-EXTRACT® MULTI-VIT B (LGE)
Art. No.: P183 / P183B
Intended Use
Immunoaffinity columns for use in conjunction with an HPLC or LC-MS/MS system for detection of biotin, folic acid and vitamin B12 in a wide range of commodities.
General Information
The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. Improved clean-up and concentration of vitamins from complex food matrices results in reduced chromatography interference and lower detection limits.
The columns contain a gel suspension of monoclonal antibody specific to the vitamins of interest. The immunoaffinity columns are designed with an integrated reservoir and a tightly fitting top and bottom cap. The top frit is absent from the top of the antibody gel to enable more thorough mixing of the sample extract and the antibody within the column. Following extraction of the vitamin the sample extract is filtered and the filtrate is added to the reservoir. The column is then placed on a rotary shaker where the sample mixes directly with the gel allowing binding to take place between the vitamin and the antibody. After allowing the gel to settle the sample is drained slowly from the column. The column is washed to remove any unbound material and the vitamin is then released from the column following elution with solvent. The eluate is collected, evaporated and reconstituted prior to analysis by HPLC.
Specifications
| Art. No. | P183 / P183B |
| Test Format | 10 large reservoir format immunoaffinity columns (P183). 50 large reservoir format immunoaffinity columns (P183B). |
| Detection Limit | LOD: Biotin – 25 ng/ml Folic Acid – 10 ng/ml Vitamin B12 – 5 ng/ml |
Contact Info
E-mail: info@chakmartin.com
Tel: 02-559-3936
Mobile: 089-892-2997
Folic Acid
EASI-EXTRACT® FOLIC ACID
Art. No.: P81 / P81B
Intended Use
Immunoaffinity columns for use in conjunction with an HPLC or LC/MS-MS for detection of folic acid in a wide range of commodities.
General Information
The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. Improved clean-up and concentration of toxins from complex food matrices results in reduced chromatography interference and lower detection limits.
The columns contain a gel suspension of monoclonal antibody specific to the vitamin of interest. Following extraction of the vitamin the sample extract is diluted with buffer, centrifuged and the supernatant filtered before being passed slowly through the immunoaffinity column. Any vitamin which is present in the sample is retained by the antibody within the gel suspension. The column is washed to remove unbound material and the vitamin is then released from the column following elution with solvent. The eluate is collected prior to analysis by HPLC or LC-MS/MS.
Specifications
| Art. No. | P81 / P81B |
| Test Format | 10 wide format immunoaffinity columns (P81). 50 wide format immunoaffinity columns (P81B). |
| Incubation Time | Total: 2 hours 20 minutes |
| Detection Limit | LOD: 10 ng/ml |
Contact Info
E-mail: info@chakmartin.com
Tel: 02-559-3936
Mobile: 089-892-2997
EASI-EXTRACT® MULTI-VIT B (LGE)
Art. No.: P183 / P183B
Intended Use
Immunoaffinity columns for use in conjunction with an HPLC or LC-MS/MS system for detection of biotin, folic acid and vitamin B12 in a wide range of commodities.
General Information
The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. Improved clean-up and concentration of vitamins from complex food matrices results in reduced chromatography interference and lower detection limits.
The columns contain a gel suspension of monoclonal antibody specific to the vitamins of interest. The immunoaffinity columns are designed with an integrated reservoir and a tightly fitting top and bottom cap. The top frit is absent from the top of the antibody gel to enable more thorough mixing of the sample extract and the antibody within the column. Following extraction of the vitamin the sample extract is filtered and the filtrate is added to the reservoir. The column is then placed on a rotary shaker where the sample mixes directly with the gel allowing binding to take place between the vitamin and the antibody. After allowing the gel to settle the sample is drained slowly from the column. The column is washed to remove any unbound material and the vitamin is then released from the column following elution with solvent. The eluate is collected, evaporated and reconstituted prior to analysis by HPLC.
Specifications
| Art. No. | P183 / P183B |
| Test Format | 10 large reservoir format immunoaffinity columns (P183). 50 large reservoir format immunoaffinity columns (P183B). |
| Incubation Time | Total: 2 hours 20 minutes |
| Detection Limit | LOD: Biotin – 25 ng/ml Folic Acid – 10 ng/ml Vitamin B12 – 5 ng/ml |
Contact Info
E-mail: info@chakmartin.com
Tel: 02-559-3936
Mobile: 089-892-2997
Multi Vitamin
– EASI-EXTRACT® MULTI-VIT B (LGE)
EASI-EXTRACT® MULTI-VIT B (LGE)
Art. No.: P183 / P183B
Intended Use
Immunoaffinity columns for use in conjunction with an HPLC or LC-MS/MS system for detection of biotin, folic acid and vitamin B12 in a wide range of commodities.
General Information
The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. Improved clean-up and concentration of vitamins from complex food matrices results in reduced chromatography interference and lower detection limits.
The columns contain a gel suspension of monoclonal antibody specific to the vitamins of interest. The immunoaffinity columns are designed with an integrated reservoir and a tightly fitting top and bottom cap. The top frit is absent from the top of the antibody gel to enable more thorough mixing of the sample extract and the antibody within the column. Following extraction of the vitamin the sample extract is filtered and the filtrate is added to the reservoir. The column is then placed on a rotary shaker where the sample mixes directly with the gel allowing binding to take place between the vitamin and the antibody. After allowing the gel to settle the sample is drained slowly from the column. The column is washed to remove any unbound material and the vitamin is then released from the column following elution with solvent. The eluate is collected, evaporated and reconstituted prior to analysis by HPLC.
Specifications
| Art. No. | P183 / P183B |
| Test Format | 10 large reservoir format immunoaffinity columns (P183). 50 large reservoir format immunoaffinity columns (P183B). |
| Detection limit | LOD: Biotin – 25 ng/ml Folic Acid – 10 ng/ml Vitamin B12 – 5 ng/ml |
Contact Info
E-mail: info@chakmartin.com
Tel: 02-559-3936
Mobile: 089-892-2997
Vitamin B12
– EASI-EXTRACT® VITAMIN B12 (LGE)
EASI-EXTRACT® VITAMIN B12 (LGE)
Art. No.: P88 / P88B
Intended Use
Novel immunoaffinity columns with built-in reservoir for detection of vitamin B12 in a wide of range commodities, developed in conjunction with Nestle Research Centre, Switzerland.
General Information
The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. Improved clean-up and concentration of toxins from complex food matrices results in reduced chromatography interference and lower detection limits.
The columns contain a gel suspension of monoclonal antibody specific to the vitamin of interest. The immunoaffinity columns are designed with an integrated reservoir and a tightly fitting top and bottom cap. The top frit is absent from the top of the antibody gel to enable more thorough mixing of the sample extract and the antibody within the column. Following extraction of the vitamin the sample extract is filtered and the filtrate is added to the reservoir. The column is then placed on a rotary shaker where the sample mixes directly with the gel allowing binding to take place between the vitamin and the antibody. After allowing the gel to settle the sample is drained slowly from the column. The column is washed to remove any unbound material and the vitamin is then released from the column following elution with solvent. The eluate is collected, evaporated and reconstituted prior to analysis by HPLC.
Certificates
AOAC Official First Action Method – 2011.08
Specifications
| Art. No. | P88 / P88B |
| Test Format | 110 large reservoir format immunoaffinity columns (P88). 50 large reservoir format immunoaffinity columns (P88B). |
| Detection limit | LOD: 5 ng/ml. |
| Approvals | Columns have been validated and approved by Nestlé. Received AOAC Official First Action Method 2011.08. |
Contact Info
E-mail: info@chakmartin.com
Tel: 02-559-3936
Mobile: 089-892-2997
Your Content Goes Here
Art. No.: P80 / P80B
Intended Use
Immunoaffinity columns for use in conjunction with an HPLC or LC-MS/MS system for detection of vitamin B12 in a wide range of commodities.
General Information
The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. Improved clean-up and concentration of toxins from complex food matrices results in reduced chromatography interference and lower detection limits.
The columns contain a gel suspension of monoclonal antibody specific to the vitamin of interest. Following extraction of the vitamin the sample extract is diluted with buffer and filtered before being passed slowly through the immunoaffinity column. Any vitamin which is present in the sample is retained by the antibody within the gel suspension. The column is washed to remove any unbound material and the vitamin is then released from the column following elution with solvent. The eluate is collected, evaporated and reconstituted prior to analysis by HPLC or LC-MS/MS.
Specifications
Art. No.: P80 / P80B
Test format: 10 wide format immunoaffinity columns (P80).
50 wide format immunoaffinity columns (P80B).
Detection limit: LOD: 0.0015 µg/injection
Contact Info
E-mail: info@chakmartin.com
Tel: 02-559-3936
Mobile: 089-892-2997
– EASI-EXTRACT® MULTI-VIT B (LGE)
EASI-EXTRACT® MULTI-VIT B (LGE)
Art. No.: P183 / P183B
Intended Use
Immunoaffinity columns for use in conjunction with an HPLC or LC-MS/MS system for detection of biotin, folic acid and vitamin B12 in a wide range of commodities.
General Information
The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. Improved clean-up and concentration of vitamins from complex food matrices results in reduced chromatography interference and lower detection limits.
The columns contain a gel suspension of monoclonal antibody specific to the vitamins of interest. The immunoaffinity columns are designed with an integrated reservoir and a tightly fitting top and bottom cap. The top frit is absent from the top of the antibody gel to enable more thorough mixing of the sample extract and the antibody within the column. Following extraction of the vitamin the sample extract is filtered and the filtrate is added to the reservoir. The column is then placed on a rotary shaker where the sample mixes directly with the gel allowing binding to take place between the vitamin and the antibody. After allowing the gel to settle the sample is drained slowly from the column. The column is washed to remove any unbound material and the vitamin is then released from the column following elution with solvent. The eluate is collected, evaporated and reconstituted prior to analysis by HPLC.
Specifications
| Art. No. | P183 / P183B |
| Test Format | 10 large reservoir format immunoaffinity columns (P183).
50 large reservoir format immunoaffinity columns (P183B). |
| Detection Limit | LOD: Biotin – 25 ng/ml
Folic Acid – 10 ng/ml Vitamin B12 – 5 ng/ml |
Contact Info
E-mail: info@chakmartin.com
Tel: 02-559-3936
Mobile: 089-892-2997
Accessories
– VitaFast® Folsäure/Folic Acid Dotierstandard/Spiking Standard
VitaFast® Folsäure/Folic Acid Dotierstandard/Spiking Standard
Intended Use
Folic acid standard for the use of spiking food, feed and pharmaceutical products.
Specifications
Art. No.: P3001
Test format: 3 x folic acid spiking standard (solid)
Contact Info
E-mail: info@chakmartin.com
Tel: 02-559-3936
Mobile: 089-892-2997
– VitaFast® Vitamin B7 (Biotin) Dotierstandard/Spiking Standard
VitaFast® Vitamin B7 (Biotin) Dotierstandard/Spiking Standard
Intended Use
Biotin standard for the use of spiking food, feed and pharmaceutical products.
Specifications
Art. No.: P3003
Test format: 3 x biotin spiking standard (solid)
Contact Info
E-mail: info@chakmartin.com
Tel: 02-559-3936
Mobile: 089-892-2997
– VitaFast® Pantothensäure/Pantothenic acid Dotierstandard/Spiking Standard
VitaFast® Pantothensäure/Pantothenic acid Dotierstandard/Spiking Standard
Intended Use
Ca – pantothenate – standard for the use of spiking food, feed and pharmaceutical products.
Specifications
Art. No.: P3005
Test format: 3 x Ca – pantothenate spiking standard (solid)
Contact Info
E-mail: info@chakmartin.com
Tel: 02-559-3936
Mobile: 089-892-2997
– VitaFast® Vitamin B12 (Cyanocobalamin) Dotierstandard/Spiking Standard
VitaFast® Vitamin B12 (Cyanocobalamin) Dotierstandard/Spiking Standard
Intended Use
Vitamin B12 standard for the use of spiking food, feed and pharmaceutical products.
Specifications
Art. No.: P3002
Test format: 3 x vitamin B12 (cyanocobalamin) spiking standard (solid)
Contact Info
E-mail: info@chakmartin.com
Tel: 02-559-3936
Mobile: 089-892-2997